Many beekeepers believe that you must remove attendant bees from queen shipping cages before you introduce a caged queen into a hive. They believe the queen will more likely be killed by the receiving hive if both the attendants and the queen have a foreign odor.
This simply is not true. If you install the caged queen properly, the attendants will cause no problem. Before long the queen’s pheromone will circulate throughout the hive. All the bees—as well as the attendants—will then smell the same.
You can install the queen and her attendants by simply putting the shipping cage near the center of the brood nest or cluster. For best results, the hive should have been queenless for at least 24 hours prior to installation. You can then just stick the shipping cage into the wax comb on one of the frames with the screen side open to the bees. Make sure the candy end is up and the cork end is down.
After several days, the worker bees will chew away the candy plug and release the queen into the hive. By then, the pheromone will be well distributed and the attendant workers will be absorbed into the colony along with the queen.
The bigger risk to the queen—especially by inexperienced beekeepers—may result from trying to get the attendants out of the queen cage. Queens have been lost, injured, or killed by well-meaning beekeepers who wrongly believed the attendants were a threat.
For more information on queen introduction, Strachan Apiaries, Inc. has a succinct little write-up on its website. Their instruction sheet specifically states that it is not necessary to remove the attendants. And you can trust them. After all, they are in the business of providing quality queens to beekeepers . . . and they don’t want them destroyed.